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Mandrika and colleagues (Biochem Pharmacol, 2001, PMID 11239505) examined melanocortin peptides on NF-κB DNA binding in RAW 264.7 macrophage-like cells and reported evidence for dual mechanisms of action, and Kannengiesser and colleagues (Inflamm Bowel Dis, 2008, PMID 18092346) reported effects in murine colitis models that were partly independent of MC1R

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As noted above, they have been utilized to unravel complex PCET reaction mechanisms