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The 0.01 mg/kg dose that showed consistent effects in rats translates to approximately 0.0016 to 0.004 mg/kg in humans, which works out to roughly 0.112 to 0.28 mg for a 154-pound person

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After washing three times with TBST (Tris-buffered saline supplemented with 0.1% Tween-20), the samples were incubated for 1 hour at room temperature with a secondary antibody