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Palmitic acid, lauric acid, myristic acid, and hydrogenated fats in HLD increase the SubG1 phase by arresting the G0/G1 phase, loss of mitochondrial potential, and decreasing viability of hepatocytes and cardiomyocytes, respectively, which further indicates programmed cell death of hepatocytes and cardiomyocytes
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Cell viability evaluated by MTT assay Viability of the cells was assayed using cell proliferation Kit I with the absorbance of 595 nm being detected using a Perkin-Elmer Victor plate reader